Fluorescence PCR · HPV
HPV16 and HPV18 Detection Kit (Fluorescence PCR)
For the in vitro qualitative detection of human papillomavirus (HPV) types 16 and 18 nucleic acids in cervical exfoliated cells. This assay aids in the screening of high-risk HPV infections associated with cervical cancer.
- SKU Code
AENE-CC001- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
For the in vitro qualitative detection of human papillomavirus (HPV) types 16 and 18 nucleic acids in cervical exfoliated cells. This assay aids in the screening of high-risk HPV infections associated with cervical cancer.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | HPV |
Epidemiology
Cervical cancer ranks among the most frequent malignancies of the female reproductive system. Persistent and multiple HPV infections have been identified as major contributors to cervical cancer. At present, universally accepted effective therapies for HPV-induced cervical cancer remain elusive. Hence, early identification and prevention of HPV infection are crucial to avert malignant transformation. The development of straightforward, specific, and swift pathogen detection assays is vitally important for the clinical diagnosis of cervical cancer.
Work Flow
- Option 1: Use Sample Release Reagent according to the provided instructions.
- Option 2: Employ a general DNA/RNA kit and an automatic nucleic acid extractor following the manufacturer's guidelines. Use a sample volume of 200 µL and an elution volume of 80 µL.
- Option 3: Utilize a commercial DNA purification kit (e.g., silica-based technology) strictly adhering to the manufacturer's protocol. Use 200 µL of sample and elute in 80 µL.
| SKU code | AENE-CC001 |
|---|---|
| Storage | ≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | cervical exfoliated cell |
| Ct | ≤28 |
| CV | ≤10.0% |
| LoD | 500 Copies/mL |
| Specificity | When use the kit to test non-specific specimens that may cross-react with its targets, all results are negative, including Ureaplasma urealyticum, Chlamydia trachomatis, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mildew, Gardnerella and other HPV types not coverd by the kit. |
| Applicable Instruments | SLAN ® -96P Real-Time PCR Systems Applied Biosystems 7500 Real-Time PCR Systems QuantStudio ® 5 Real-Time PCR Systems LightCycler ® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler. |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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