Fluorescence PCR · Respiratory Infections
11-Plex Respiratory Pathogen Nucleic Acid Detection Kit (Fluorescence PCR)
This multiplex fluorescence PCR kit qualitatively detects 11 clinically relevant respiratory pathogens: Haemophilus influenzae, Streptococcus pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Stenotrophomonas maltophilia, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophila, from human sputum. It aids diagnosis of bacterial respiratory infections in hospitalized or critically ill patients.
- SKU Code
AENE-RT162A- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
This multiplex fluorescence PCR kit qualitatively detects 11 clinically relevant respiratory pathogens: Haemophilus influenzae, Streptococcus pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Stenotrophomonas maltophilia, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophila, from human sputum. It aids diagnosis of bacterial respiratory infections in hospitalized or critically ill patients.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Respiratory Infections |
Epidemiology
Respiratory tract infections represent a major category of diseases that pose a severe health risk. Studies have demonstrated that the majority of these infections arise from co-infections involving bacterial and/or viral pathogens, which heighten disease severity and can result in death. Consequently, pinpointing the responsible pathogen facilitates targeted therapeutic approaches and boosts patient survival rates [1,2].
Work Flow
Using a universal DNA/RNA extraction kit (which can be operated alongside an automated nucleic acid extractor), introduce 200 µL of physiological saline to the processed precipitate, and then follow the kit’s protocol. The advised elution volume is 100 µL.
| SKU code | AENE-RT162A |
|---|---|
| Storage | ≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Sputum |
| Ct | HI、SP、KPN、PA、ABA、Smet：Ct≤33 BP、Bpp、MP、Cpn、Leg：Ct≤38 |
| CV | 5.0% |
| LoD | Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, Pseudomonas aeruginosa, Acinetobacter baumannii, Stenotrophomonas maltophilia and Legionella pneumophila: 1000 CFU/mL ； Bordetella pertussis and Bacillus parapertussis: 500 CFU/mL; Mycoplasma pneumoniae and Chlamydia pneumoniae: 200 copies/mL. |
| Specificity | The cross-reactivity test results showed that there was no cross reaction between this kit and Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, attenuated strains of Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii and human genomic nucleic acids. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio ® 5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler ® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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