Fluorescence PCR · Respiratory Infections
2019-nCoV/Influenza A/Influenza B/RSV Nucleic Acid Detection Kit (Fluorescence PCR)
For qualitative detection of 2019-nCoV, influenza A, influenza B, and respiratory syncytial virus nucleic acids in human oropharyngeal swabs to aid in the diagnosis of respiratory infections.
- SKU Code
AENE-RT099- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
For qualitative detection of 2019-nCoV, influenza A, influenza B, and respiratory syncytial virus nucleic acids in human oropharyngeal swabs to aid in the diagnosis of respiratory infections.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Respiratory Infections |
Epidemiology
Coronavirus Disease 2019 (COVID-19) is pneumonia caused by the 2019-nCoV virus, a β genus coronavirus. It is an acute respiratory infectious disease to which the general population is susceptible. Currently, infected patients are the principal source, though asymptomatic individuals can also transmit the virus. Epidemiological data indicate an incubation period of 1–14 days, typically 3–7 days. Common symptoms include fever, dry cough, and fatigue, with some experiencing nasal congestion, runny nose, sore throat, muscle pain, and diarrhea.
Work Flow
Option 1: Use a viral DNA/RNA extraction kit with an automatic nucleic acid extractor. Process 200 µL of sample and elute in 80 µL.
Option 2: Use a viral RNA mini kit (such as a column-based method) or a nucleic acid extraction/purification kit. Process 140 µL of sample and elute in 60 µL.
| SKU code | AENE-RT099 |
|---|---|
| Storage | -18℃ |
| Shelf-life | 9 months |
| Specimen Type | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Copies/mL Influenza A virus/Influenza B virus/Respiratory syncytial virus: 500Copies/mL |
| Specificity | a) Cross-reactivity results show that there is no cross reaction between the kit and human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, human pulmonary virus, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci and newborn cryptococcus and human genomic nucleic acid. b) Anti-interference ability: select mucin (60mg/mL), 10% (v/v) of blood and phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (including preservatives) (20 mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir(500mg/mL), ritonavir(60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) and tobramycin (0.6mg/mL) for interference test, and the results show that interfering substanc |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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