Fluorescence PCR · Respiratory Infections
4 Kinds of Respiratory Viruses Nucleic Acid Detection Kit (Fluorescence PCR)
Qualitative detection of SARS-CoV-2, influenza A/B, and RSV nucleic acids in oropharyngeal swabs. Aids in diagnosis of COVID-19 and other respiratory infections.
- SKU Code
AENE-RT186- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
Qualitative detection of SARS-CoV-2, influenza A/B, and RSV nucleic acids in oropharyngeal swabs. Aids in diagnosis of COVID-19 and other respiratory infections.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Respiratory Infections |
Epidemiology
COVID-19, short for Corona Virus Disease 2019, is the pneumonia resulting from infection with SARS-CoV-2, a β-genus coronavirus. This acute respiratory illness can affect the general population. The primary infection sources are individuals with SARS-CoV-2 infection, though asymptomatic carriers may also transmit the virus. Epidemiological data indicate an incubation period of 1 to 14 days, most commonly 3 to 7 days. Typical symptoms include fever, dry cough, and fatigue; some patients also experience nasal congestion, runny nose, sore throat, myalgia, or diarrhea.
Influenza, often called the flu, is an acute respiratory infection caused by influenza viruses. It is highly contagious and spreads mainly through coughing and sneezing, with seasonal outbreaks typically in spring and winter. Influenza viruses are classified into three types: A (IFV A), B (IFV B), and C (IFV C). All are sticky viruses that primarily cause human disease via types A and B, which are single-stranded, segmented RNA viruses. Influenza A virus causes acute respiratory infections and includes subtypes like H1N1 and H3N2; it is known for frequent mutations and global outbreaks. The term "shift" describes a mutation in influenza A that generates a new subtype. Influenza B viruses are split into two lineages—Yamagata and Victoria. They undergo only antigenic drift, using mutations to evade immune detection, though they evolve more slowly than influenza A. Influenza B can also lead to respiratory infections and epidemics.
Respiratory syncytial virus (RSV) is an RNA virus from the paramyxoviridae family. It spreads via airborne droplets and close contact, serving as a major cause of lower respiratory tract infections in infants. RSV infection in infants may result in severe bronchiolitis and pneumonia, conditions linked to childhood asthma. Infants often exhibit high fever, rhinitis, pharyngitis, and laryngitis, which can progress to bronchiolitis and pneumonia. Some children may develop complications like otitis media, pleurisy, or myocarditis. In adults and older children, the infection typically presents as an upper respiratory tract illness.
Work Flow
Two options are available for extraction:
- Option 1: Use the Viral DNA/RNA Kit() in conjunction with an Automatic Nucleic Acid Extractor.
- Option 2: Use the Nucleic Acid Extraction or Purification Reagent (YDP302).
| SKU code | AENE-RT186 |
|---|---|
| Storage | 2-8°C |
| Shelf-life | 12 months |
| Specimen Type | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | SARS-CoV-2: 150Copies/mL Influenza A virus/Influenza B virus/Respiratory syncytial virus: 300Copies/mL |
| Specificity | There is no cross-reactivity with human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, human pulmonary virus, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s. pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci and newborn cryptococcus and human genomic nucleic acid. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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