Fluorescence PCR · Respiratory Infections
Adenovirus Universal Detection Kit (Fluorescence PCR)
For qualitative detection of adenovirus nucleic acid in nasopharyngeal swab and throat swab samples, aiding in the diagnosis of adenovirus respiratory infections.
- SKU Code
AENE-RT017A- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
For qualitative detection of adenovirus nucleic acid in nasopharyngeal swab and throat swab samples, aiding in the diagnosis of adenovirus respiratory infections.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Respiratory Infections |
Epidemiology
Human adenovirus (HAdV) is a non-enveloped, double-stranded DNA virus belonging to the Mammalian adenovirus genus. Currently, 67 types are classified into seven subgroups (A–G), of which 55 serotypes are pathogenic to humans. Respiratory tract infections are primarily caused by group B (types 3, 7, 11, 14, 16, 21, 50, 55), group C (types 1, 2, 5, 6, 57), and group E (type 4), while group F (types 40 and 41) mainly causes intestinal diarrhea [1-8]. Clinical presentations differ by type, but respiratory illness is most common. Adenovirus-associated respiratory diseases account for 5%–15% of global respiratory diseases and 5%–7% of childhood respiratory diseases worldwide [9]. Adenovirus is endemic globally, circulates throughout the year, and frequently causes localized outbreaks in crowded settings such as schools and military barracks.
Work Flow
Recommended extraction reagent: Sample Release Reagent. Follow the manufacturer's instructions. Add the patient's nasopharyngeal or throat swab, collected on site, to the Sample Release Reagent, vortex thoroughly, leave at room temperature for 5 minutes, then invert and mix to obtain DNA from each sample.
Recommended extraction reagent: Viral DNA/RNA Kit and Automatic Nucleic Acid Extractor. Perform the extraction exactly as specified in the instructions. Process a 200 μL sample volume; elute in 80 μL.
Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent (YDP315). Strictly adhere to the manufacturer's protocol. Use a 200 μL sample and elute in the recommended volume of 80 μL.
| SKU code | AENE-RT017A |
|---|---|
| Storage | ≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Nasopharyngeal swab ， Throat swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300Copies/mL |
| Specificity | a) Test the standardized company negative references by the kit, and the test result meets the requirements. b) Use this kit to detect and there is no cross-reactivity with other respiratory pathogens (such as Influenza A virus, Influenza B virus, Respiratory syncytial virus, Parainfluenza virus, Rhinovirus, Human metapneumovirus, etc.) or bacteria (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, etc.). |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co. Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System s ( FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co. Ltd.) BioRad CFX96 Real-Time PCR Systems, BioRad CFX Opus 96 Real-Time PCR Systems |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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