Fluorescence PCR · Respiratory Infections
Eight Kinds of Respiratory Viruses Nucleic Acid Detection Kit (Fluorescence PCR)
Qualitative detection of influenza A virus (IFV A), influenza B virus (IFVB), respiratory syncytial virus (RSV), adenovirus (Adv), human metapneumovirus (hMPV), rhinovirus (Rhv), parainfluenza virus (PIV), and Mycoplasma pneumoniae (MP) nucleic acids in oropharyngeal and nasopharyngeal swab samples.
- SKU Code
AENE-RT184- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
Qualitative detection of influenza A virus (IFV A), influenza B virus (IFVB), respiratory syncytial virus (RSV), adenovirus (Adv), human metapneumovirus (hMPV), rhinovirus (Rhv), parainfluenza virus (PIV), and Mycoplasma pneumoniae (MP) nucleic acids in oropharyngeal and nasopharyngeal swab samples.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Respiratory Infections |
Epidemiology
Respiratory tract infections represent the most prevalent human illnesses, affecting individuals regardless of gender, age, or geographic location, and stand as a leading contributor to global morbidity and mortality [1]. Frequently encountered respiratory pathogens include influenza A virus (IFV A), influenza B virus (IFV B), respiratory syncytial virus, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus types I/II/III, and Mycoplasma pneumoniae, among others [2,3]. Although the clinical presentations induced by these infections are often similar, the therapeutic approaches, outcomes, and disease progression vary depending on the causative pathogen [4,5]. Current laboratory diagnostic methods for respiratory pathogens encompass viral isolation, antigen detection, and nucleic acid amplification testing. This assay is intended for the qualitative detection and identification of specific viral nucleic acids in specimens from patients exhibiting signs and symptoms of respiratory infection, serving as an adjunct to other clinical and laboratory findings to support the diagnosis of viral respiratory disease.
Work Flow
Two formats of the Viral DNA/RNA Kit are provided: one compatible with the Automatic Nucleic Acid Extractor, and another suitable for manual extraction workflows. The recommended sample input volume is 200 μL, and the suggested elution volume is 150 μL.
| SKU code | AENE-RT184 |
|---|---|
| Storage | 2-8℃ |
| Shelf-life | 12 months |
| Specimen Type | Oropharyngeal swab; Nasopharyngeal swab |
| Ct | IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | 5.0% |
| LoD | 200 Copies/mL |
| Specificity | Cross reactivity: There is no cross reactivity between the kit and Boca virus, Cytomegalovirus, Epstein-Barr virus, Herpes simplex virus, varicella zoster Virus, Mumps virus, Enterovirus, measles virus, human coronavirus, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Lactobacillus, Corynebacterium, human genomic DNA. Interference test: Select mucin (60mg/mL), human blood (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL), sodium chloride with 5% preservative (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), fluniacetone (20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L), The results showed that the interfering substances at the above concentra |
| Applicable Instruments | Applicable to type I detection reagent: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio ® 5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co. Ltd.), LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co. Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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