Fluorescence PCR · Respiratory Infections
Freeze-dried 11-plex Respiratory Pathogen Detection Kit (Fluorescence PCR)
Qualitative detection of 11 common bacterial respiratory pathogens in human sputum, including Haemophilus influenzae, Streptococcus pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Stenotrophomonas maltophilia, Bordetella pertussis, Bacillus parapertussis, Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophila, to aid diagnosis of respiratory tract infections in hospitalized or critically ill patients.
- SKU Code
AENE-RT190- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
Qualitative detection of 11 common bacterial respiratory pathogens in human sputum, including Haemophilus influenzae, Streptococcus pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Stenotrophomonas maltophilia, Bordetella pertussis, Bacillus parapertussis, Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophila, to aid diagnosis of respiratory tract infections in hospitalized or critically ill patients.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Respiratory Infections |
Epidemiology
Infections of the respiratory tract pose a serious global health threat. Research indicates that the majority of these infections result from co-infection by bacteria and/or viruses, which can worsen disease severity and increase mortality. Accurate pathogen identification therefore allows for targeted therapy and improved survival outcomes[1,2]. Conventional detection techniques—such as microscopic examination, bacterial culture, and immunological assays—are often laborious, time-consuming, technically challenging, and lack sensitivity. Moreover, they typically cannot identify multiple pathogens in a single sample, hindering precise diagnostic support for clinicians. Consequently, treatment frequently remains empirical, accelerating bacterial resistance and delaying appropriate patient care[3]. The following organisms are important causes of nosocomial respiratory infections: Haemophilus influenzae, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Stenotrophomonas maltophilia, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophila[4,5]. This assay detects the specific nucleic acids of these pathogens in individuals presenting with respiratory infection signs and symptoms, and, when integrated with other laboratory findings, aids in the diagnosis of respiratory pathogen infections.
Work Flow
For sample extraction, a Type I viral DNA/RNA purification kit (compatible with automatic nucleic acid extraction systems) is recommended. All subsequent steps must be performed in strict accordance with the kit's instructions for use.
| SKU code | AENE-RT190 |
|---|---|
| Storage | 2-30℃ |
| Shelf-life | 12 months |
| Specimen Type | Throat swab |
| Ct | ≤33 |
| CV | 5.0% |
| LoD | The LoD of the kit for Klebsiella pneumoniae is 500 CFU/mL; the LoD of Streptococcus pneumoniae is 500 CFU/mL; the LoD of Haemophilus influenzae is 1000 CFU/mL; the LoD of Pseudomonas aeruginosa is 500 CFU/mL; the LoD of Acinetobacter baumannii is 500 CFU/mL; the LoD of Stenotrophomonas maltophilia is 1000 CFU/mL; the LoD of Bordetella pertussis is 500 CFU/mL; the LoD of Bordetella parapertussis is 500 CFU/mL; the LoD of Mycoplasma pneumoniae is 200 copies/mL; the LoD of Legionella pneumophila is 1000 CFU/mL; the LoD of Chlamydia pneumoniae is 200 copies/mL. |
| Specificity | There is no cross reaction between the kit and other common respiratory pathogens outside the detection range of the test kit, e.g. Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, Candida albicans, Klebsiella oxytoca, Streptococcus pyogenes, Micrococcus luteus, Rhodococcus equi, Listeria monocytogenes, Acinetobacter junii, Haemophilus parainfluenzae, Legionella dumov, Enterobacter aerogenes, Haemophilus haemolyticus, Streptococcus salivarius, Neisseria meningitidis, Mycobacterium tuberculosis, Influenza A virus, Influenza B virus, Aspergillus flavus, Aspergillus terreus, Aspergillus fumigatus, Candida glabrata, and Candida tropicalis. |
| Applicable Instruments | Type I: Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio ® 5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co. Ltd.), LightCycler ® 480 Real-Time PCR Systems, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co. Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. Type II: |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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