Fluorescence PCR · Respiratory Infections
Freeze-dried Influenza A/B Virus Nucleic Acid Detection Kit (Fluorescence PCR)
The kit is intended for the in vitro qualitative detection of influenza A virus (IFV A) and influenza B virus (IFV B) RNA in human nasopharyngeal swab samples. It aids in the differential diagnosis of influenza A and B infections.
- SKU Code
AENE-RT193- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
The kit is intended for the in vitro qualitative detection of influenza A virus (IFV A) and influenza B virus (IFV B) RNA in human nasopharyngeal swab samples. It aids in the differential diagnosis of influenza A and B infections.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Respiratory Infections |
Epidemiology
Influenza viruses are classified into four types based on antigenic variations in the NP and M genes: influenza A (IFV A), influenza B (IFV B), influenza C (IFV C), and influenza D (IFV D). Influenza A virus has a broad host range, numerous serotypes, and can cross species barriers through genetic reassortment and adaptive mutations. Because humans do not acquire lasting immunity against influenza A, individuals of all ages are susceptible, making it the primary cause of influenza pandemics. In contrast, influenza B virus typically spreads locally and lacks distinct subtypes; human infections are predominantly associated with the B/Yamagata and B/Victoria lineages. In the Asia-Pacific region, monthly confirmed influenza cases across 15 countries show influenza B diagnosis rates varying from 0% to 92%. Unlike influenza A, certain populations—including children and the elderly—are particularly vulnerable to influenza B, which can lead to severe complications and may impose a greater societal burden than influenza A.
Work Flow
For conventional PCR, a general DNA/RNA kit, compatible with an automatic nucleic acid extractor, is recommended for sample extraction. All subsequent steps must be performed in strict accordance with the kit’s instructions for use. Alternatively, an all-in-one machine may be employed for integrated processing.
| SKU code | AENE-RT193 |
|---|---|
| Storage | 2-28 ℃ |
| Shelf-life | 12 months |
| Specimen Type | Throat swab |
| Ct | IFV A ， IFVB Ct≤35 |
| CV | 5.0% |
| LoD | 200 Copies/mL |
| Specificity | Cross-reactivity: There is no cross-reactivity between the kit and Bocavirus, Rhinovirus, Cytomegalovirus, Respiratory syncytial virus, Parainfluenza virus, Epstein-Barr virus, herpes simplex virus, varicella-zoster virus, Mumps virus, Enterovirus, Measles virus, human metapneumovirus, Adenovirus, human coronaviruses, novel coronavirus, SARS-CoV, MERS-CoV, Rotavirus, Norovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis jirovecii, Haemophilus influenzae, Bordetella pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, Neisseria gonorrhoeae, Candida albicans, Candida glabrata, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium and human genomic DNA. Interference test: Mucin (60mg/mL), human blood (50%), Phenylephrine (2 mg/mL), Oxymetazoline (2mg/mL), Sodium chloride (20mg/mL) with 5% preservative, Beclomethasone (20mg/mL), Dexamethasone (20mg/mL), Flunisolide (20μg/mL), Triamcinolone (2mg/mL), Budesonide (1mg/mL), Mometasone (2mg/mL), Fluticasone (2mg/mL), Histamine hydrochloride (5 mg/mL), Benzocaine (10%), Menthol (10%), Zanamivir (20mg/mL), Peramivir (1mg/mL), Mupirocin (20mg/mL), Tobramycin (0.6mg/mL), Oseltamivir (60ng/mL), Ribavirin (10mg/L) were selected for the interference test, and the results showed that the interfering substances at the above concentrations had no interference react |
| Applicable Instruments | Applicable to Type I test reagent: Applied Biosystems 7500 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co. Ltd.). Applicable to Type II test reagent: |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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