Fluorescence PCR · Respiratory Infections
Influenza A Virus/Influenza B Virus Detection Kit (Fluorescence PCR)
This kit is used for the qualitative detection of influenza A virus and influenza B virus RNA in human oropharyngeal swab samples.
- SKU Code
AENE-RT174- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
This kit is used for the qualitative detection of influenza A virus and influenza B virus RNA in human oropharyngeal swab samples.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Respiratory Infections |
Epidemiology
Influenza viruses are categorized into four types—influenza A, B, C, and D—based on antigenic variation in the NP and M genes [1]. Influenza A virus exhibits a broad host range, numerous serotypes, and the capacity to cross species barriers through genetic recombination and adaptive mutations. Since humans do not develop durable immunity against it, all age groups are generally susceptible, and it is the principal agent behind influenza pandemics [2]. Influenza B virus typically circulates in more circumscribed areas and lacks distinct subtypes; the two lineages responsible for most human infections are B/Yamagata and B/Victoria. Among confirmed influenza cases in 15 Asia-Pacific countries, the monthly proportion attributed to influenza B virus ranged from 0% to 92% [3]. In contrast to influenza A, influenza B disproportionately affects children and the elderly, who are both more susceptible and more likely to suffer complications, resulting in a greater societal impact [4].
Work Flow
A viral DNA/RNA extraction kit, compatible with an automated nucleic acid extractor, is recommended for sample extraction. All subsequent steps must be strictly performed according to the kit’s instructions for use (IFU).
| SKU code | AENE-RT174 |
|---|---|
| Storage | ≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Oropharyngeal swab sample |
| Ct | Flu A, Flu B Ct≤3 5 |
| CV | 5.0% |
| LoD | Flu A and Flu B are all 200Copies/mL |
| Specificity | Cross-reactivity: There is no cross reaction between the kit and Bocavirus, rhinovirus, cytomegalovirus, respiratory syncytial virus, parainfluenza virus, Epstein-Barr virus, herpes simplex virus, Varicella-zoster virus, mumps virus, enterovirus, measles virus, human metapneumovirus, adenovirus, human coronavirus, novel coronavirus, SARS coronavirus, MERS coronavirus, rotavirus, norovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis carinii, Haemophilus influenzae, Bordetella pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, Neisseria gonorrhoeae, Candida albicans, Candida glabrata, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium, and human genomic DNA. Interference test: Select mucin (60 mg/mL), human blood (50%), phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (20mg/mL) with 5% preservative, beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L) for interference tests, and the results show that the interfering substances at the above concentrations do not interfere |
| Applicable Instruments | SLAN-96P Real-Time PCR Systems Applied Biosystems 7500 Real-Time PCR System QuantStudio ® 5 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
Send the essentials for a useful first reply.
Share the application, sample type, format, destination, and expected quantity so AeneBio can route AENE-RT174 to the right quote or sample discussion.