Fluorescence PCR · Respiratory Infections
Influenza A Virus (Universal/H1/H3) Detection Kit (Fluorescence PCR)
Qualitative detection of influenza A virus universal, H1, and H3 nucleic acids in human nasopharyngeal swabs using fluorescence PCR, aiding in the diagnosis of influenza A virus infections.
- SKU Code
AENE-RT012- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
Qualitative detection of influenza A virus universal, H1, and H3 nucleic acids in human nasopharyngeal swabs using fluorescence PCR, aiding in the diagnosis of influenza A virus infections.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Respiratory Infections |
Epidemiology
Belonging to the Orthomyxoviridae family, influenza viruses pose a significant threat to human health with their broad host range. Seasonal epidemics impact roughly 600 million individuals globally, resulting in 250,000 to 500,000 deaths, primarily caused by influenza A virus. This virus possesses a single-stranded negative-sense RNA genome. Based on the surface proteins hemagglutinin (HA) and neuraminidase (NA), HA types are classified into 16 subtypes and NA into 9 subtypes. Among influenza A viruses, subtypes known to directly infect humans include A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2, and H10N8. The H1 and H3 subtypes are highly pathogenic and warrant special attention.
Work Flow
Method 1: Use a nucleic acid extraction or purification reagent. Follow the manufacturer’s instructions strictly. The sample volume is 140 μL, and the recommended elution volume is 60 μL.
Method 2: Combine a viral DNA/RNA kit with an automatic nucleic acid extractor. Adhere closely to the kit’s protocol. Use a sample volume of 200 μL, and elute with 80 μL.
| SKU code | AENE-RT012 |
|---|---|
| Storage | ≤-18℃ |
| Shelf-life | 9 months |
| Specimen Type | nasopharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Copies/μL |
| Specificity | No cross-reactivity with other respiratory samples such as Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, etc. and human genomic DNA. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co. Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co. Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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