Fluorescence PCR · Respiratory Infections
Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Carbapenem Resistance Genes (KPC, NDM, OXA48, IMP) Multiplex Detection Kit (Fluorescence PCR)
For qualitative in vitro detection of Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and carbapenem resistance genes (KPC, NDM, OXA48, IMP) in human sputum. Supports clinical diagnosis and treatment decisions for suspected bacterial infections.
- SKU Code
AENE-RT109- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
For qualitative in vitro detection of Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and carbapenem resistance genes (KPC, NDM, OXA48, IMP) in human sputum. Supports clinical diagnosis and treatment decisions for suspected bacterial infections.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Respiratory Infections |
Certificate
CE
Epidemiology
Klebsiella pneumoniae is a prevalent opportunistic pathogen in clinical environments and a leading cause of nosocomial infections. When host defenses weaken, the bacterium can enter the lungs through the respiratory tract, leading to infections in multiple body sites; prompt antibiotic treatment is essential for a cure [1]. The primary site of Acinetobacter baumannii infection is the lungs, making it a major pathogen in hospital-acquired pneumonia (HAP), especially ventilator-associated pneumonia (VAP). It frequently coexists with other bacteria and fungi, contributing to high morbidity and mortality rates. Pseudomonas aeruginosa is the most common non-fermentative Gram-negative bacillus in clinical settings and a significant opportunistic agent in hospital-acquired infections, characterized by easy colonization, a high mutation rate, and multidrug resistance.
Work Flow
For sample extraction, the General DNA/RNA Kit (compatible with an Automatic Nucleic Acid Extractor) is recommended. All subsequent steps must be performed in strict accordance with the kit's instructions for use (IFU).
| SKU code | AENE-RT109 |
|---|---|
| Storage | ≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Sputum |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 copies/mL |
| Specificity | a)The cross-reactivity test shows that this kit has no cross reactivity with other respiratory pathogens, such as Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus and sputum samples without targets, etc. b)Anti-interference ability: Select mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, and roxithromycin, etc. for the interference test, and the results show that the interference substances mentioned above do not interfere with the detection of Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and carbapenem resistance genes KPC, NDM, OXA48 and IMP. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio ® 5 Real-Time PCR Systems, LightCycler ® 480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co. Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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