Fluorescence PCR · Respiratory Infections
Legionella pneumophila Nucleic Acid Detection Kit (Fluorescence PCR)
Qualitative detection of Legionella pneumophila nucleic acid in sputum samples to aid in the diagnosis of Legionella pneumophila infection.
- SKU Code
AENE-RT163- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
Qualitative detection of Legionella pneumophila nucleic acid in sputum samples to aid in the diagnosis of Legionella pneumophila infection.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Respiratory Infections |
Epidemiology
Legionella pneumophila is a gram-negative, flagellated, short coccobacillus belonging to the polymorphic genus Legionella. This facultative intracellular bacterium can invade both amoebae and human macrophages. Its infectivity is significantly boosted by antibodies and serum complement, although neither is strictly required. As a major cause of epidemic and sporadic community-acquired pneumonia as well as hospital-acquired pneumonia, it accounts for roughly 80% of Legionella pneumonia cases. The organism primarily resides in water and soil, and transmission occurs mainly through inhalation of contaminated aerosols. Laboratory diagnosis traditionally relies on bacterial culture and serological testing.
Work Flow
A Viral DNA/RNA Kit is available for use with an Automatic Nucleic Acid Extractor or in manual protocols. The extraction requires a 200 µL sample volume, with a recommended elution volume of 150 µL.
| SKU code | AENE-RT163 |
|---|---|
| Storage | -18℃ |
| Shelf-life | 12 months |
| Specimen Type | sputum |
| Ct | ≤38 |
| CV | 5.0% |
| LoD | 1000 Copies/μL |
| Applicable Instruments | Applicable to type I detection reagent: Applied Biosystems 7500 Real-Time PCR Systems,QuantStudio®5 Real-Time PCR Systems,SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co. Ltd.),LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology),MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co. Ltd.),BioRad CFX96 Real-Time PCR System,BioRad CFX Opus 96 Real-Time PCR System. |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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