Fluorescence PCR · Respiratory Infections
Mycoplasma pneumoniae (MP) Detection Kit (Fluorescence PCR)
For in vitro qualitative detection of Mycoplasma pneumoniae (MP) nucleic acid in sputum and oropharyngeal swab samples, aiding the diagnosis of MP-associated respiratory tract infections, including atypical pneumonia in children and young adults.
- SKU Code
AENE-RT024- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
For in vitro qualitative detection of Mycoplasma pneumoniae (MP) nucleic acid in sputum and oropharyngeal swab samples, aiding the diagnosis of MP-associated respiratory tract infections, including atypical pneumonia in children and young adults.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Respiratory Infections |
Epidemiology
Mycoplasma pneumoniae (MP) is one of the smallest prokaryotic organisms, lying between bacteria and viruses. It has a cellular structure but no cell wall. MP primarily causes respiratory infections in humans, particularly in children and young adults. The illnesses it triggers include mycoplasma pneumonia, pediatric respiratory tract infections, and atypical pneumonia. Clinical symptoms vary widely, with severe cough, fever, chills, headache, and sore throat being common. Upper respiratory tract infections and bronchial pneumonia are the most frequent manifestations. In some instances, an upper respiratory infection can advance to severe pneumonia, potentially leading to severe respiratory distress and even death.
Work Flow
Sputum Sample
Two extraction reagent options are recommended. First option: Viral DNA/RNA Kit (compatible with an automatic nucleic acid extractor). Add 200 µL of normal saline to the processed precipitate, then proceed with extraction according to the kit's instructions, using an elution volume of 80 µL. Second option: Nucleic Acid Extraction or Purification Reagent (YDP315-R). Perform extraction strictly following its instructions, with an elution volume of 60 µL.
Oropharyngeal Swab
Two extraction reagent options are recommended. First option: Viral DNA/RNA Kit (for use with an automatic nucleic acid extractor). Follow the kit's instructions, using a sample extraction volume of 200 µL and an elution volume of 80 µL. Second option: A silica membrane-based viral RNA extraction kit or Nucleic Acid Extraction or Purification Reagent (YDP315-R). Adhere strictly to the respective product's instructions, with a sample volume of 140 µL and elution volume of 60 µL.
| SKU code | AENE-RT024 |
|---|---|
| Storage | ≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Sputum、Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 200 Copies/mL |
| Specificity | a） Cross reactivity: there is no cross reactivity with Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacterium tuberculosis, Legionella pneumophila, Pseudomonas aeruginosa, Acinetobacter baumannii, Influenza A virus, Influenza B virus, Parainfluenza virus type I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus and human genomic nucleic acid. b）Anti-interference ability: there is no interference when the interfering substances were tested with the following concentrations: hemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) human blood, levofloxacin (10μg/mL), moxifloxacin(0.1g/L), gemifloxacin (80μg/mL), azithromycin (1mg/mL), clarithromycin(125μg/mL), erythromycin(0.5g/L), doxycycline (50mg/L), minocycline (0.1g/L). |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio ® 5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co. Ltd.) LightCycler ® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co. Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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