Fluorescence PCR · Respiratory Infections
Nine Respiratory Viruses Nucleic Acid Detection Kit (Fluorescence PCR)
Qualitative detection of nine respiratory pathogens (influenza A/B, SARS-CoV-2, RSV, adenovirus, hMPV, rhinovirus, parainfluenza 1/2/3, Mycoplasma pneumoniae) in oropharyngeal/nasopharyngeal swabs for aid in diagnosis of respiratory infections.
- SKU Code
AENE-RT185A- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
Qualitative detection of nine respiratory pathogens (influenza A/B, SARS-CoV-2, RSV, adenovirus, hMPV, rhinovirus, parainfluenza 1/2/3, Mycoplasma pneumoniae) in oropharyngeal/nasopharyngeal swabs for aid in diagnosis of respiratory infections.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Respiratory Infections |
Epidemiology
Respiratory tract infections represent the most prevalent human illnesses, affecting individuals irrespective of gender, age, or geographic location, and are among the leading global causes of morbidity and mortality [1]. Frequent respiratory pathogens encountered in clinical settings comprise influenza A virus (IFV A), influenza B virus (IFV B), SARS-CoV-2, respiratory syncytial virus, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus types I, II, and III, and Mycoplasma pneumoniae [2,3]. Although the clinical presentations of respiratory infections are largely similar, the therapeutic approaches, outcomes, and disease progression vary depending on the causative pathogen [4,5]. Current laboratory diagnostic techniques for respiratory pathogens include viral isolation, antigen detection, and nucleic acid testing. This assay identifies specific viral nucleic acids in patients exhibiting respiratory infection signs and symptoms, and, together with other clinical and laboratory findings, aids in diagnosing respiratory viral infections.
Work Flow
Nucleic acid extraction is performed using a viral DNA/RNA purification kit that is compatible with automated nucleic acid extraction systems as well as manual processing. The required sample input volume is 200 μL, and the suggested elution volume is 150 μL.
| SKU code | AENE-RT185A |
|---|---|
| Storage | 2-8℃ |
| Shelf-life | 12 months |
| Specimen Type | Oropharyngeal swab; Nasopharyngeal swab |
| Ct | COVID-9, IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | 5.0% |
| LoD | 200 Copies/mL |
| Specificity | Cross reactivity: There is no cross reactivity between the kit and Boca virus, Cytomegalovirus, Epstein-Barr virus, Herpes simplex virus, varicella zoster Virus, Mumps virus, Enterovirus, measles virus, human coronavirus, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Lactobacillus, Corynebacterium, human genomic DNA. Interference test: Select mucin (60mg/mL), human blood (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL), sodium chloride with 5% preservative (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), fluniacetone (20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L), The results showed that the interfering substances at the above concentration had no interfering reaction to the detection of the kit. |
| Applicable Instruments | Applicable to type I detection reagent: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio ® 5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co. Ltd.), LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co. Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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