Fluorescence PCR · Respiratory Infections
Respiratory Pathogens Combined Detection Kit (Fluorescence PCR)
Qualitative multiplex detection of SARS-CoV-2, influenza A, influenza B, influenza A H1N1, and respiratory syncytial virus (RSV) nucleic acids in oropharyngeal and nasopharyngeal swabs. Aids in the differential diagnosis of COVID-19, influenza, and RSV infections.
- SKU Code
AENE-RT183- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
Qualitative multiplex detection of SARS-CoV-2, influenza A, influenza B, influenza A H1N1, and respiratory syncytial virus (RSV) nucleic acids in oropharyngeal and nasopharyngeal swabs. Aids in the differential diagnosis of COVID-19, influenza, and RSV infections.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Respiratory Infections |
Epidemiology
Coronavirus disease 2019 (COVID-19) is pneumonia caused by SARS-CoV-2, a β‑genus coronavirus. The population is generally susceptible to this acute respiratory infection. Sources of infection are mainly patients infected with 2019‑nCoV, though asymptomatic carriers can also transmit the virus. Epidemiological data indicate an incubation period of 1 to 14 days, most commonly 3 to 7 days. Predominant symptoms are fever, dry cough, and fatigue; some patients also present with nasal congestion, runny nose, sore throat, myalgia, and diarrhea. Influenza, commonly known as the flu, is an acute, highly contagious respiratory disease caused by influenza viruses. Transmission occurs via coughing and sneezing, with outbreaks typically in spring and winter. Influenza viruses are grouped into types A (IFV A), B (IFV B), and C (IFV C). All are single‑stranded, segmented RNA orthomyxoviruses, but human disease is primarily due to types A and B. Influenza A includes H1N1, H3N2, and other subtypes that frequently mutate and cause global epidemics. A “shift” denotes a major genetic change in influenza A resulting in a new subtype. Influenza B is divided into two lineages, Yamagata and Victoria. It undergoes only antigenic drift, evading immune surveillance through mutation, though its evolution is slower than that of influenza A. Influenza B can also give rise to respiratory epidemics.
Respiratory syncytial virus (RSV) is an RNA virus in the Paramyxoviridae family, transmitted by airborne droplets and close contact. It is the principal pathogen of lower respiratory tract infections in infants. Infants infected with RSV may develop severe bronchiolitis and pneumonia, conditions associated with childhood asthma. In infants, symptoms are often severe: high fever, rhinitis, pharyngitis, laryngitis, followed by bronchiolitis and pneumonia. A minority of cases may be complicated by otitis media, pleurisy, or myocarditis. In adults and older children, RSV infection primarily causes an upper respiratory tract illness.
Work Flow
The Viral DNA/RNA Kit, compatible with an Automatic Nucleic Acid Extractor, is utilized. The extraction uses a sample volume of 200 μL, and the recommended elution volume is 150 μL.
| SKU code | AENE-RT183 |
|---|---|
| Storage | -18℃ In dark |
| Shelf-life | 9 months |
| Specimen Type | Oropharyngeal swab; Nasopharyngeal swab |
| Ct | IFV A, IFVB, RSV, SARS-CoV-2, IFV A H1N1 Ct≤3 8 |
| CV | ≤5% |
| LoD | 200 Copies/mL |
| Specificity | Cross-reactivity results show that there is no cross reaction between the kit and cytomegalovirus, herpes simplex virus type 1, varicella zoster virus, Epstein-Barr virus, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus type I/II/III/IV, bocavirus, enterovirus, coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Bordetella pertussis, Corynebacterium spp. Escherichia coli, Haemophilus influenzae, Lactobacillus spp. Legionella pneumophila, Moraxella catarrhalis, attenuated strains of Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria spp. Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium fasciatum, Nocardia, Serratia marcescens, Citrobacter rodentium, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis carinii, Candida albicans, Roseburia mucosa, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia Q fever and human genomic nucleic acid. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio ® 5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co. Ltd.), LightCycler ® 480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co. Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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