Fluorescence PCR · COVID-19
SARS-CoV-2, Influenza A, and Influenza B Detection Kit (Fluorescence PCR)
For in vitro qualitative detection of SARS-CoV-2, influenza A, and influenza B nucleic acids in nasopharyngeal and oropharyngeal swab specimens from individuals suspected of respiratory infection with these viruses.
- SKU Code
AENE-RT060A- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
For in vitro qualitative detection of SARS-CoV-2, influenza A, and influenza B nucleic acids in nasopharyngeal and oropharyngeal swab specimens from individuals suspected of respiratory infection with these viruses.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | COVID-19 |
Certificate
AKL/TGA/CE
Epidemiology
COVID-19 is an acute respiratory illness caused by the SARS-CoV-2 virus, a member of the β coronavirus genus. The population is broadly susceptible. Infected individuals are the primary source of transmission, though asymptomatic carriers can also spread the virus. Epidemiological studies indicate an incubation period ranging from 1 to 14 days, typically 3 to 7 days. Common symptoms include fever, dry cough, and fatigue; less frequent manifestations consist of nasal congestion, runny nose, sore throat, muscle pain, and diarrhea.
Influenza is an acute respiratory infection caused by influenza viruses. It spreads readily via coughing and sneezing, with peak activity typically in spring and winter. There are three types: A (IFV A), B (IFV B), and C (IFV C), all belonging to the Orthomyxoviridae family. Influenza A and B, which possess single-stranded, segmented RNA genomes, are responsible for most human disease. Influenza A virus includes subtypes such as H1N1 and H3N2 and is prone to sudden, major genetic changes known as antigenic shift, which can produce a novel viral subtype and trigger a pandemic. Influenza B virus is classified into two lineages, Yamagata and Victoria, and undergoes only gradual antigenic drift. Through these mutations, influenza B evades immune surveillance, though it evolves more slowly than influenza A; nonetheless, it still causes respiratory illness and seasonal outbreaks in humans.
Work Flow
For sample extraction, two methods are suggested:
- Option 1: Use a Viral DNA/RNA Kit combined with an automatic nucleic acid extractor.
- Option 2: Use a nucleic acid extraction or purification reagent.
| SKU code | AENE-RT060A |
|---|---|
| Storage | Liquid: ≤-18℃ In dark |
| Shelf-life | Liquid: 9 months |
| Specimen Type | Nasopharyngeal swabs, Oropharyngeal swabs |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300 Copies/mL |
| Specificity | The cross test results showed that the kit was compatible with human coronavirus SARSr- CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, respiratory syncytial virus A and B, parainfluenza virus 1, 2 and 3, rhinovirusA, B and C, adenovirus 1, 2, 3, 4, 5, 7 and 55, human metapneumovirus, enterovirus A, B, C and D, human cytoplasmic pulmonary virus, EB virus, measles virus Human cytomegalovirus, rotavirus, norovirus, mumps virus, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata There was no cross reaction between Pneumocystis yersini and Cryptococcus neoformans. |
| Applicable Instruments | : It can match the mainstream fluorescent PCR instruments on the market. Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
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