Freeze-dried Chlamydia trachomatis Detection Kit (Isothermal Amplification)

Epidemiology

Chlamydia trachomatis (CT) is a prokaryotic organism that lives exclusively as an intracellular parasite within eukaryotic hosts[1]. Based on serotyping, it is classified into serotypes A through K. Urogenital infections predominantly arise from the trachoma biovar serotypes D–K. In males, the primary presentation is urethritis, which may resolve spontaneously but often evolves into a chronic state with episodic exacerbations, and can be associated with epididymitis, proctitis, and related conditions[2]. In females, possible manifestations include urethritis and cervicitis, with the more severe complication being salpingitis[3].

Work Flow

Option 1

Using Sample Release Reagent, extraction must follow the manufacturer's instructions precisely. Combine the DNA obtained via sample release reagent with the reaction buffer and immediately analyze on the instrument; alternatively, hold extracted samples at 2–8°C for up to 24 hours.

Option 2

With a General DNA/RNA Kit and an Automatic Nucleic Acid Extractor, adhere strictly to the IFU during extraction; the suggested elution volume is 80 µL. DNA recovered by the magnetic bead method should be heated at 95°C for 3 minutes, then promptly chilled on an ice bath for 2 minutes. Add the treated DNA to the reaction buffer and proceed with testing, or keep processed samples below -18°C for no longer than 4 months, with a maximum of 4 freeze–thaw cycles.