Fluorescence PCR · Antibiotic Resistance
Carbapenem Resistance Genes (KPC/NDM/OXA-48/OXA-23/VIM/IMP) Detection Kit (Fluorescence PCR)
Qualitative detection of carbapenem resistance genes including KPC (Klebsiella pneumoniae carbapenemase), NDM (New Delhi metallo-β-lactamase 1), OXA-48 (oxacillinase 48), OXA-23 (oxacillinase 23), VIM (Verona Imipenemase), and IMP (Imipenemase) in human sputum, rectal swabs, and pure colonies.
- SKU Code
AENE-OT045- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
Qualitative detection of carbapenem resistance genes including KPC (Klebsiella pneumoniae carbapenemase), NDM (New Delhi metallo-β-lactamase 1), OXA-48 (oxacillinase 48), OXA-23 (oxacillinase 23), VIM (Verona Imipenemase), and IMP (Imipenemase) in human sputum, rectal swabs, and pure colonies.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Antibiotic Resistance |
Epidemiology
Carbapenems represent an atypical class of β-lactam antibiotics, known for their exceptionally broad spectrum and potent antibacterial effects. Their stability against β-lactamases and low toxicity have established them as crucial agents for managing severe bacterial infections. These antibiotics exhibit high resistance to plasmid-mediated extended-spectrum β-lactamases (ESBLs), chromosomal enzymes, and plasmid-mediated cephalosporinases (AmpC enzymes).
Work Flow
Option 1.
Recommended extraction reagent: General DNA/RNA Kit (compatible with an Automatic Nucleic Acid Extractor). Add 200 μL of normal saline to the bacterial pellet. Follow the extraction instructions for subsequent steps, with a recommended elution volume of 100 μL.
Option 2.
Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent (YDP302). Begin extraction strictly according to step 2 of the instructions for use: add 200 μL of buffer GA to the bacterial pellet, and shake until the pellet is completely suspended. Use RNase/DNase-free water for elution, with a recommended elution volume of 100 μL.
Option 3.
Recommended extraction reagent: Sample Release Reagent. For sputum samples, wash the previously treated bacterial pellet by adding 1 mL of normal saline, centrifuge at 13000 rpm for 5 minutes, and discard the supernatant (leaving 10–20 µL of supernatant). For pure colonies and rectal swabs, add 50 μL of sample release reagent directly to the treated bacterial pellet, and then proceed with extraction according to the instructions for use.
| SKU code | AENE-OT045 |
|---|---|
| Storage | ≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Sputum, pure colonies, rectal swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 10 3 CFU/mL |
| Specificity | a) The kit detects the standardized company negative references, and the results meet the requirements of the corresponding references. b) The results of the cross-reactivity test show that this kit has no cross reaction with other respiratory pathogens, such as Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, or samples containing other drug-resistant genes CTX, mecA, SME, SHV, TEM, etc. c) Anti-interference: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin are selected for interference test, and the results show that the above-mentioned interfering substances have no interfering reaction to the detection of carbapenem resistance genes KPC, NDM, OXA48, OXA23, VIM, and IMP. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co. Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System ( FQD-96A ， Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co. Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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