Fluorescence PCR · Antibiotic Resistance
Vancomycin-Resistant Enterococcus and VanA/VanB Drug-Resistant Genes Detection Kit (Fluorescence PCR)
Qualitative detection of vancomycin-resistant Enterococcus (VRE) and the VanA/VanB drug-resistance genes in human sputum, blood, urine, or pure colonies.
- SKU Code
AENE-OT090- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
Qualitative detection of vancomycin-resistant Enterococcus (VRE) and the VanA/VanB drug-resistance genes in human sputum, blood, urine, or pure colonies.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Antibiotic Resistance |
Epidemiology
Drug resistance, also termed antibacterial resistance, is the ability of bacteria to withstand the effects of antibacterial agents. When resistance develops, the therapeutic efficacy of chemotherapy is substantially reduced. Resistance is classified as either intrinsic or acquired. Intrinsic resistance is determined by chromosomal genes that are inherited and remain stable over generations. Acquired resistance results from bacteria altering their metabolic pathways after antibiotic exposure so they can survive the drug’s effects.
The vancomycin resistance genes VanA and VanB are types of acquired drug resistance. VanA confers high levels of resistance to both vancomycin and teicoplanin, whereas VanB leads to varying levels of vancomycin resistance while remaining sensitive to teicoplanin. Vancomycin is frequently used to treat Gram-positive bacterial infections; however, the rise of vancomycin-resistant enterococci (VRE), especially enterococcus faecalis and enterococcus faecium (which account for over 90% of cases), has created significant challenges for clinical therapy. Currently, no specific antibacterial drug exists for VRE treatment. Moreover, VRE can transfer resistance genes to other enterococci or other Gram-positive bacteria.
Work Flow
Recommended extraction reagents: a genomic DNA kit and an automatic nucleic acid extractor.
| SKU code | AENE-OT090 |
|---|---|
| Storage | Liquid: ≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | sputum, blood, urine or pure colonies |
| CV | ≤5.0% |
| Ct | ≤36 |
| LoD | 10 3 CFU/mL |
| Specificity | There is no cross-reactivity with other respiratory pathogens such as klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxytoca, haemophilus influenzae, A. junii, A. haemolyticus, legionella pneumophila, escherichia coli, pseudomonas fluorescens, candida albicans, chlamydia pneumoniae, respiratory adenovirus, or samples contain other drug-resistant genes CTX, mecA, SME, Samples of SHV and TEM. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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