Fluorescence PCR · Gastrointestinal
Enterovirus 71 (EV71) Detection Kit (Fluorescence PCR)
Intended for the in vitro qualitative detection of Enterovirus 71 (EV71) nucleic acid in oropharyngeal swabs and herpes fluid samples from patients with hand-foot-mouth disease.
- SKU Code
AENE-EV003- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
Intended for the in vitro qualitative detection of Enterovirus 71 (EV71) nucleic acid in oropharyngeal swabs and herpes fluid samples from patients with hand-foot-mouth disease.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Gastrointestinal |
Epidemiology
Hand, foot, and mouth disease (HFMD) is triggered by enteroviruses, of which 108 serotypes have been identified and classified into groups A through D. The primary causative agents are enterovirus 71 (EV71) and coxsackievirus A16 (CoxA16). The illness predominantly affects children younger than 5 years, presenting with vesicular lesions on the hands, feet, and oral mucosa. A minority of cases may progress to severe complications such as myocarditis, pulmonary edema, and aseptic meningoencephalitis.
Work Flow
Option 1.
For on-site sample collection, use a Sample Release Reagent following the provided directions. Collect oropharyngeal swabs or herpes fluid specimens directly into the reagent. Vortex the mixture thoroughly, incubate at room temperature for 5 minutes, and then invert to mix well. This process extracts the RNA from each sample.
Option 2.
Use a General DNA/RNA Kit (suitable for use with an automatic nucleic acid extractor) and perform extraction as instructed. Use 200 µL of sample and elute in a recommended volume of 80 µL.
Option 3.
Employ a commercial viral RNA mini kit or a virus DNA/RNA extraction kit (catalog no. YDP315-R), adhering strictly to the kit's protocol. Use a sample volume of 140 µL and elute in 60 µL.
| SKU code | AENE-EV003 |
|---|---|
| Storage | ≤-18℃ |
| Shelf-life | 9 months |
| Specimen Type | Oropharyngeal swab， Herpes fluid |
| Ct | ≤35 |
| CV | 5.0% |
| LoD | 500Copies/mL |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio ® 5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler ® 480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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