Fluorescence PCR · Hepatitis
Hepatitis A Virus Detection Kit (Fluorescence PCR)
Qualitative detection of hepatitis A virus (HAV) nucleic acid in serum and stool specimens. In vitro diagnostic use.
- SKU Code
AENE-HP005- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
Qualitative detection of hepatitis A virus (HAV) nucleic acid in serum and stool specimens. In vitro diagnostic use.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Hepatitis |
Epidemiology
Acute viral hepatitis is primarily caused by the hepatitis A virus (HAV). HAV is a positive-sense, single-stranded RNA virus belonging to the Hepadnavirus genus of the Picornaviridae family. The virus is mainly transmitted via the fecal-oral route and resists heat, acids, and many organic solvents, allowing prolonged survival in shellfish, water, soil, and seabed sediments [1-3]. Infection occurs through ingestion of contaminated food or water, or by direct person-to-person contact. Foods often linked to HAV outbreaks include oysters, clams, strawberries, raspberries, blueberries, dates, leafy greens, and semi-dried tomatoes [4‒6].
Work Flow
Serum samples
Option 1: Use the General DNA/RNA Kit () with the Automatic Nucleic Acid Extractor. Perform extraction following the kit instructions; the recommended elution volume is 80 µL.
Option 2: Use the TIANamp Virus DNA/RNA Kit (YDP315-R). Follow the protocol provided with the kit. Process a sample volume of 140 μL and elute in 60 μL.
Stool samples
Use the General DNA/RNA Kit () and the Automatic Nucleic Acid Extractor. Carry out extraction as directed by the instructions; the suggested elution volume is 80 µL.
| SKU code | AENE-HP005 |
|---|---|
| Storage | ≤-18℃ |
| Shelf-life | Liquid: 9 months, Lyophilized: 12 months |
| Specimen Type | Serum/stool |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 2 Copies/μL |
| Specificity | Use the kits to test other hepatitis viruses such as hepatitis B, C, D, E, enterovirus 71, coxsackie virus, Epstein-Barr virus, norovirus, HIV and human genome.There is no cross-reactivity. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio ® 5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co. Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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