Fluorescence PCR · Hepatitis
Hepatitis B Virus DNA Detection Kit (Fluorescence PCR)
Quantitative detection of hepatitis B virus (HBV) DNA in human serum or plasma. Aids in diagnosis and monitoring of HBV infection.
- SKU Code
AENE-HP015- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
Quantitative detection of hepatitis B virus (HBV) DNA in human serum or plasma. Aids in diagnosis and monitoring of HBV infection.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Hepatitis |
Epidemiology
Hepatitis B, caused by the hepatitis B virus (HBV), is primarily marked by liver inflammation and can affect multiple organs. Clinical manifestations include fatigue, anorexia, edema of the lower limbs or whole body, and enlarged liver due to hepatic dysfunction. Among adults infected, 5% are unable to clear the virus, while in vertical transmission, 95% become persistently infected. Chronic infection may eventually lead to cirrhosis and hepatocellular carcinoma [1–4].
Work Flow
The recommended extraction uses a virus DNA/RNA kit compatible with an automated nucleic acid extractor. Follow the manufacturer’s instructions: starting sample volume is 300 µL and the elution volume is 70 µL.
| SKU code | AENE-HP015 |
|---|---|
| Storage | ≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | fresh serum、Plasma |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 5 IU/mL |
| Specificity | The specificity results show that all 50 cases of healthy HBV DNA negative serum samples are negative; the cross-reactivity test results show that there is no cross-reaction between this kit and other viruses (HAV, HCV, DFV, HIV) for nucleic acid detection with blood samples, and human genomes. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co. Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co. Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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