Fluorescence PCR · Oncology
Human BRAF Gene V600E Mutation Detection Kit (Fluorescence PCR)
Qualitatively detects the BRAF V600E mutation in formalin-fixed paraffin-embedded (FFPE) tissue specimens from human melanoma, colorectal cancer, thyroid cancer, and lung cancer using fluorescence PCR technology.
- SKU Code
AENE-TM007- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
Qualitatively detects the BRAF V600E mutation in formalin-fixed paraffin-embedded (FFPE) tissue specimens from human melanoma, colorectal cancer, thyroid cancer, and lung cancer using fluorescence PCR technology.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Oncology |
Certificate
CE/TFDA
Epidemiology
Over 30 distinct BRAF mutations have been identified, with approximately 90% occurring in exon 15. The most prevalent is the V600E mutation, where a thymine (T) to adenine (A) change at nucleotide 1799 leads to a valine (V) to glutamic acid (E) substitution at codon 600. These mutations are frequently observed in malignancies such as melanoma, colorectal carcinoma, thyroid cancer, and lung cancer. In the context of targeted therapy, assessing BRAF mutation status is essential for identifying patients who may respond to EGFR-TKIs and BRAF-targeted agents.
Work Flow
Recommended extraction reagents: a DNA FFPE tissue kit and a rapid paraffin-embedded tissue DNA extraction kit.
| SKU code | AENE-TM007 |
|---|---|
| Storage | ≤-18℃ |
| Shelf-life | 9 months |
| Specimen Type | paraffin-embedded pathological tissue samples |
| CV | ＜5.0% |
| Ct | ≤38 |
| LoD | Use the kits to detect the corresponding LoD quality control. a) under 3ng/μL wild-type background, 1% mutation rate can be detected in the reaction buffer stably; b) under 1% mutation rate, the mutation of 1×10 3 Copies/mL in the wild-type background of 1×10 5 Copies/mL can be stably detected in the reaction buffer; c) the IC Reaction Buffer can detect the lowest detection limit quality control SW3 of the company internal control. |
| Applicable Instruments | : Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7300 Real-Time PCR Systems, QuantStudio® 5 Real-Time PCR Systems LightCycler®480 Real-Time PCR system BioRad CFX96 Real-Time PCR System |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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