Fluorescence PCR · Oncology
Human PML-RARA Fusion Gene Detection Kit (Fluorescence PCR)
Qualitative detection of PML-RARA fusion gene transcripts in human bone marrow by fluorescence PCR, aiding in the diagnosis and monitoring of acute promyelocytic leukemia (APL).
- SKU Code
AENE-TM017A- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
Qualitative detection of PML-RARA fusion gene transcripts in human bone marrow by fluorescence PCR, aiding in the diagnosis and monitoring of acute promyelocytic leukemia (APL).
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Oncology |
Epidemiology
Acute promyelocytic leukemia (APL) is a distinct subtype of acute myeloid leukemia (AML). Approximately 95% of APL patients carry a characteristic cytogenetic alteration, the t(15;17)(q22;q21) translocation, which fuses the PML gene on chromosome 15 with the retinoic acid receptor α gene (RARA) on chromosome 17 to generate the PML-RARA fusion gene. Based on the breakpoint within the PML gene, the fusion transcript is classified as long (L), short (S), or variant (V) type, occurring at rates of about 55%, 40%, and 5%, respectively.
Work Flow
The recommended reagent for RNA extraction is a blood total RNA purification kit. Perform the extraction in accordance with the kit's instructions for use.
| SKU code | AENE-TM017A |
|---|---|
| Storage | ≤-18℃ In dark |
| Shelf-life | 9 months |
| Specimen Type | bone marrow |
| CV | 5.0％ |
| LoD | 1000 Copies/mL. |
| Specificity | There is no cross-reactivity with other fusion genes BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, and TEL-AML1 fusion genes |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio ® 5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co. Ltd.) LightCycler ® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co. Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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