Orientia tsutsugamushi Detection Kit (Fluorescence PCR)

Qualitative detection of Orientia tsutsugamushi nucleic acid in serum samples by fluorescence PCR, used as an aid in the diagnosis of scrub typhus.

SKU Code
AENE-OT002B
Approval
On request
Product Type
Assay / Kit
Status
Available

Qualitative detection of Orientia tsutsugamushi nucleic acid in serum samples by fluorescence PCR, used as an aid in the diagnosis of scrub typhus.

Product typeAssay / Kit
Technology platformFluorescence PCR
Application areaOthers

Epidemiology

Scrub typhus is an acute febrile disease caused by Orientia tsutsugamushi (Ot), a Gram-negative obligate intracellular bacterium. It belongs to the genus Orientia within the family Rickettsiaceae, order Rickettsiales. Transmission primarily occurs through the bite of infected chigger larvae. Clinically, it presents with sudden high fever, an eschar, lymphadenopathy, hepatosplenomegaly, and peripheral blood leukopenia. Severe cases can progress to meningitis, hepatic and renal failure, multi-organ failure, and even death.

Work Flow

Recommended extraction reagent: a general DNA/RNA kit (compatible with an automatic nucleic acid extractor). Follow the kit instructions for extraction. Use a sample volume of 200 µL and elute with 100 µL.

SKU codeAENE-OT002B
Storage≤-18℃
Shelf-life12 months
Specimen Typefresh serum
Tt≤38
CV≤5.0%
LoD500 Copies/μL
Applicable InstrumentsApplied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio ® 5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co. Ltd.) LightCycler ® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co. Ltd.) BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System
ApprovalOn request
DocumentsDatasheet, IFU, SDS, COA / conformance, origin, and export documents where available

FAQ

What should be confirmed before quotation?

Sample context, collection method, and extraction workflow should be confirmed before final kit selection.

Which documents can be requested?

Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.

Can the format be adjusted for different workflows?

Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.

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