Fluorescence PCR · Arbovirus
Dengue Virus, Zika Virus and Chikungunya Virus Multiplex Detection Kit (Fluorescence PCR)
This kit is used for the qualitative detection of dengue virus, Zika virus and chikungunya virus nucleic acids in serum samples.
- SKU Code
AENE-FE040- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
This kit is used for the qualitative detection of dengue virus, Zika virus and chikungunya virus nucleic acids in serum samples.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Arbovirus |
Epidemiology
Dengue fever (DF), caused by infection with the dengue virus (DENV), is a highly prevalent arboviral illness. The disease is transmitted by mosquitoes of the species Aedes aegypti and Aedes albopictus and occurs mainly in tropical and subtropical regions. DENV is a flavivirus in the family Flaviviridae and can be grouped into four serotypes based on surface antigens. Common symptoms of infection include headache, fever, malaise, enlarged lymph nodes, and leukopenia; severe cases may involve hemorrhage, shock, liver damage, or death. Factors such as climate change, urbanization, and increased travel have accelerated the spread of DF, resulting in a continual expansion of its endemic areas.
Work Flow
Option 1.
Extraction using the TIANamp Virus DNA/RNA Kit (YDP315-R) must be performed strictly as directed. The starting sample volume is 140 µL, and the advised elution volume is 60 µL.
Option 2.
Extraction with the General DNA/RNA Kit, which is usable with the Automatic Nucleic Acid Extractor, should follow its instructions. The sample volume is 200 µL, with a recommended elution volume of 80 µL.
| SKU code | AENE-FE040 |
|---|---|
| Storage | -18℃ |
| Shelf-life | 9 months |
| Specimen Type | Fresh serum |
| Ct | ≤38 |
| CV | ＜ 5 % |
| LoD | 500 Copies/mL |
| Specificity | Interference test results show that when the concentration of bilirubin in serum is not more than 168.2μmol/ml, the hemoglobin concentration produced by hemolysis is not more than 130g/L, the blood lipid concentration is not more than 65mmol/ml, the total IgG concentration in serum is not more than 5mg/mL, there is no effect on the dengue virus, Zika virus or chikungunya virus detection. Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Herpes virus, Eastern equine encephalitis virus, Hantavirus, Bunya virus, West Nile virus and human genomic serum samples are selected for the cross-reactivity test, and the results show that there is no cross reaction between this kit and the pathogens mentioned above. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio ® 5 Real-Time PCR Systems LightCycler ® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
Send the essentials for a useful first reply.
Share the application, sample type, format, destination, and expected quantity so AeneBio can route AENE-FE040 to the right quote or sample discussion.