Fluorescence PCR · Meningitis
Forest Encephalitis Virus Detection Kit (Fluorescence PCR)
This kit is a real-time fluorescent PCR assay for qualitative detection of forest encephalitis virus (tick-borne encephalitis virus) RNA in serum. It aids in the diagnosis of tick-borne encephalitis, a central nervous system infection.
- SKU Code
AENE-FE006- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
This kit is a real-time fluorescent PCR assay for qualitative detection of forest encephalitis virus (tick-borne encephalitis virus) RNA in serum. It aids in the diagnosis of tick-borne encephalitis, a central nervous system infection.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Meningitis |
Epidemiology
Forest encephalitis (FE), also called tick-borne encephalitis (TBE), is an acute central nervous system infection caused by the forest encephalitis virus. This virus belongs to the genus Flavivirus, family Flaviviridae. The spherical particles measure 40–50 nm in diameter, have a molecular weight near 4×106 Da, and contain a positive-sense single-stranded RNA genome [1]. Clinical manifestations include high fever, headache, coma, rapidly progressing meningeal irritation, and muscle paralysis affecting the neck and limbs, resulting in a high mortality rate. Early and rapid detection of the virus is critical for treatment, and developing a simple, specific, and quick etiological diagnostic method is highly valuable for clinical diagnosis [1,2].
Work Flow
Employ a nucleic acid extraction or purification kit (YDP315-R) and strictly follow the accompanying protocol. The recommended sample input volume is 140 µL, with an elution volume of 60 µL.
Alternatively, use a general DNA/RNA kit () together with an automatic nucleic acid extractor (). Adhere precisely to the respective instructions. For this approach, use a sample volume of 200 µL and elute in 80 µL.
| SKU code | AENE-FE006 |
|---|---|
| Storage | ≤-18℃ |
| Shelf-life | 9 months |
| Specimen Type | fresh serum |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500Copies/mL |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio ® 5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler ® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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