Fluorescence PCR · Meningitis
Xinjiang Hemorrhagic Fever Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Qualitative detection of Xinjiang hemorrhagic fever virus nucleic acid in serum from suspected patients, aiding diagnosis of Xinjiang hemorrhagic fever.
- SKU Code
AENE-FE007B/C- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
Qualitative detection of Xinjiang hemorrhagic fever virus nucleic acid in serum from suspected patients, aiding diagnosis of Xinjiang hemorrhagic fever.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Meningitis |
Epidemiology
The Xinjiang hemorrhagic fever virus was initially isolated from blood specimens of patients suffering from hemorrhagic fever in the Tarim Basin, Xinjiang, China, as well as from local hard ticks, which gave the virus its name. Clinical features encompass fever, headache, bleeding, and hypotensive shock. The core pathological alterations consist of systemic capillary dilation, congestion, enhanced permeability, and fragility, leading to variable degrees of congestion and hemorrhage in the skin, mucous membranes, and tissues of multiple organs throughout the body. Degeneration and necrosis affect solid organs such as the liver, adrenal glands, and pituitary, while a jelly-like edema develops in the retroperitoneal space.
Work Flow
One recommended extraction reagent is the Viral DNA/RNA Kit, which is compatible with automated nucleic acid extractors. The extraction procedure should follow the kit instructions, using a sample volume of 200 µL and an elution volume of 80 µL.
An alternative extraction approach employs a viral RNA mini kit paired with a nucleic acid extraction or purification reagent. In this protocol, follow the manufacturer's instructions precisely, with a sample volume of 140 µL and an elution volume of 60 µL.
| SKU code | AENE-FE007B/C |
|---|---|
| Storage | ≤-18℃ |
| Shelf-life | 9 months |
| Specimen Type | fresh serum |
| Tt | ≤38 |
| CV | ＜ 5.0% |
| LoD | 1000Copies/mL |
| Specificity | No cross-reactivity with other respiratory samples such as Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, etc. and human genomic DNA. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio ® 5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler ® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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