Fluorescence PCR · Meningitis
Zaire Ebola Virus Detection Kit (Fluorescence PCR)
This kit is intended for the qualitative detection of Zaire Ebola virus nucleic acid in serum or plasma samples from patients suspected of Zaire Ebola virus infection, aiding in the diagnosis of Ebola hemorrhagic fever.
- SKU Code
AENE-FE008- Approval
- On request
- Product Type
- Assay / Kit
- Status
- Available
This kit is intended for the qualitative detection of Zaire Ebola virus nucleic acid in serum or plasma samples from patients suspected of Zaire Ebola virus infection, aiding in the diagnosis of Ebola hemorrhagic fever.
| Product type | Assay / Kit |
|---|---|
| Technology platform | Fluorescence PCR |
| Application area | Meningitis |
Epidemiology
Ebola virus is a member of the Filoviridae family, characterized as an unsegmented, negative-sense, single-stranded RNA virus. Virions are elongated filaments, typically measuring about 1000 nm in length and 100 nm in diameter. The genome is roughly 18.9 kb in size and encodes seven structural proteins plus one non-structural protein. Five subtypes are recognized: Zaire, Sudan, Bundibugyo, Taï Forest, and Reston. The Zaire and Sudan subtypes are particularly lethal, having caused numerous human fatalities. Infection leads to Ebola hemorrhagic fever (EHF), an acute hemorrhagic illness transmitted primarily through contact with bodily fluids, secretions, or excretions from infected individuals or animals. Clinical features include high fever, hemorrhagic manifestations, and multi-organ failure. The case fatality rate ranges from 50% to 90%.
Work Flow
Option 1.
Utilize a viral RNA extraction kit or a nucleic acid purification reagent. Follow the manufacturer's instructions carefully. The recommended sample input volume is 140 µL, and the elution volume is 60 µL.
Option 2.
Use a viral DNA/RNA extraction kit paired with an automated nucleic acid extraction system. Adhere to the provided protocol. The sample volume should be 200 µL, and the elution volume is 80 µL.
Option 3.
Apply a nucleic acid extraction reagent and a high‑throughput automated sample preparation system. Extract as directed, with a sample volume of 160 µL and an elution volume of 60 µL.
| SKU code | AENE-FE008 |
|---|---|
| Storage | ≤-18℃ |
| Shelf-life | 9 months |
| Specimen Type | Fresh serum、Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Copies/μL |
| Specificity | Use the kits to test company negative references, the results meet the requirements. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co. Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System(FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co. Ltd.) BioRad CFX96 Real-Time PCR System, and BioRad CFX Opus 96 Real-Time PCR System |
| Approval | On request |
|---|---|
| Documents | Datasheet, IFU, SDS, COA / conformance, origin, and export documents where available |
FAQ
What should be confirmed before quotation?
Sample context, collection method, and extraction workflow should be confirmed before final kit selection.
Which documents can be requested?
Datasheet, IFU, SDS, COA / conformance, origin, insurance, and export documents can be checked where available.
Can the format be adjusted for different workflows?
Liquid or lyophilized format options should be confirmed together with instrument compatibility and shipment requirements.
Inquiry
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